Journal: Cell communication and signaling : CCS

Article Title: Nck1 depletion induces activation of the PI3K/Akt pathway by attenuating PTP1B protein expression.

doi: 10.1186/s12964-014-0071-9

Figure Lengend Snippet: Figure 2 EGF- and PDGF-induced Akt phosphorylation is enhanced in Nck1-depleted HepG2 cells. Overnight serum-starved HepG2 cells transfected with control or Nck1 siRNA were exposed to 0, 1 and 10 nM EGF (A) or 0, 25 and 50 ng/ml PDGF-BB (B) for 5 min. Equal amount of proteins from total cell lysates were subjected to immunoblot with indicated antibodies. Data are representative of three independent experiments performed in duplicate with similar results. (C) HepG2 cells transfected with control or different Nck1 siRNAs (Nck1-1, Nck1-2) were starved overnight. Equal amount of proteins from total cell lysates were subjected to immunoblot with indicated antibodies. Data are representative of three independent experiments showing similar results.

Article Snippet: HepG2 cells were starved overnight in MEM containing 0.1% bovine serum albumin (BSA, Invitrogen) after 48 h siRNA transfection, and then incubated with either insulin (Eli Lilly Co., Indianapolis, IN), EGF (Fitzgerald Industries International), PDGF-BB (Roche), pervanadate (Sgima), LY294002 (Calbiochem) or Akt inhibitor 1/2 (Calbiochem) at concentrations indicated in Figure legends.

Techniques: Phospho-proteomics, Transfection, Control, Western Blot

Journal: Scientific Reports

Article Title: Menstrual blood-derived mesenchymal stromal cell extracellular vesicles stimulate chondrocytes and cartilage extracellular matrix synthesis in vitro

doi: 10.1038/s41598-026-40854-3

Figure Lengend Snippet: MenSC-EV effect on chondrocyte sex hormone receptor protein and gene expression. A Estrogen receptor 1 alpha (ESR1) and progesterone receptor (PGR) protein synthesis in chondrocyte monolayer after stimulation with MenSC-EVs for 3 days (ELISA). Control – chondrocytes cultured under the same conditions, but without MenSC-EVs. Each dot represents a single donor’s result. B ESR1 and PGR gene expression in chondrocyte monolayer, after stimulation with/without MenSC-EVs for 3 days. Relative mRNA level presented after normalization to two housekeeping genes (B2M and RPS9). Fold change on y axys represent EV/control ratio. * and horizontal bar represent p ≤ 0.05 (Welch non-parametric test).

Article Snippet: PGR , Hs01556702_m1 , Progesterone receptor.

Techniques: Gene Expression, Enzyme-linked Immunosorbent Assay, Control, Cell Culture

Journal: Scientific Reports

Article Title: Menstrual blood-derived mesenchymal stromal cell extracellular vesicles stimulate chondrocytes and cartilage extracellular matrix synthesis in vitro

doi: 10.1038/s41598-026-40854-3

Figure Lengend Snippet: Gene expression analysis (RT-qPCR) of cartilage explants, after treatment with MenSC-EVs. Collagen type II (COL2A1), aggrecan (ACAN), transforming growth factor beta receptor 2 (TGFBR2), metalloproteinase 1 (MMP1), metalloproteinase 13 (MMP13), cathepsin K (CTSK), estrogen receptor 1 alpha (ESR1) and progesterone (PGR) receptor genes were evaluated in explants after 7 days in culture. Relative mRNA level presented after normalization to two housekeeping genes (B2M and RPS9). Fold change on y axis represents EV/Control ratio. * represents p ≤ 0.05 (non-parametric Mann Whithey test).

Article Snippet: PGR , Hs01556702_m1 , Progesterone receptor.

Techniques: Gene Expression, Quantitative RT-PCR, Control